Fig. 1

Reg4 promotes resistance to high-fat diet-induced obesity. a Body weight increases in male (left) or female (right) WT1 and Reg4 KO mice (R4KO) fed a high-fat diet (HFD) (n = 14). The body weights of these mice did not differ at baseline before HFD feeding. b Typical phenotypes of male WT1 and Reg4 KO mice (R4KO) fed a HFD for 3 months. c Fat pad weights of WT1 and Reg4KO mice (R4KO) fed a HFD (n = 14). d Glucose tolerance and insulin sensitivity of WT1 and Reg4 KO mice (R4KO) fed a HFD for 3 months (n = 6). e H/E staining of the adipose tissues of WT1 and Reg4 KO mice (R4KO) fed a HFD. f qRT-PCR of TNFα, IL6, and MCP-1 in the adipose tissues of WT1 and Reg4 KO mice (R4KO) fed a HFD (n = 14). g Body weight increases in male (left) and female (right) WT2 and huREG4^IECtg mice (HuR4) fed a HFD (n = 14). The body weights of these mice did not differ at baseline before HFD feeding. h Typical phenotypes of male WT2 and hu^REG4IECtg mice (HuR4) fed a HFD for 3 months. i Fat pad weights of WT2 and huREG4^IECtg mice (HuR4) fed a HFD (n = 14). j Glucose tolerance and insulin sensitivity of WT2 and huREG4^IECtg mice (HuR4) fed a HFD (n = 6). k H/E staining of the adipose tissues of WT2 and huREG4^IECtg mice (HuR4) fed a HFD. l qRT-PCR of TNFα, IL6, and MCP-1 in the adipose tissues of WT2 and huREG4^IECtg mice (HuR4) fed a HFD (n = 14). m Flow cytometry of IFNγ⁺CD4⁺, Foxp3⁺CD4⁺, F4/80⁺CD11C⁺, and F4/80⁺CD206⁺ cells in the adipose tissues of Reg4 KO (R4KO) and control WT mice (WT1) fed a HFD for 3 months. n Flow cytometry of IFNγ⁺CD4⁺, Foxp3⁺CD4⁺, F4/80⁺CD11C⁺, and F4/80⁺CD206⁺ cells in the adipose tissues of huREG4^IECtg (HuR4) and control littermate WT mice (WT2) fed a HFD for 3 months. The data in a–l are representative of three independent experiments; the data in m and n are from three independent experiments. Scale bars = 40 μm; analysis of variance in a, d, g, and j; Student’s t test in other panels, mean ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001