Fig. 4

HAMSAB supplementation is accompanied by modulation of the immune system at W6 and W12 follow-up. A Multivariate PLS-DA comparing the proportions of major immune populations assessed by mass cytometry within total live cells. Significance determined by PERMANOVA. B PLS-DA plot loadings indicating the contribution of each immune population. C Proportions of total CD19⁺ B cells and IgD⁺CD27^- naïve B cells within live cells. D IgD⁺IgM^hiCD27⁺ MZ B cells expressing CD86 (mean geometric signal intensity, MSI). E CD3⁺ T cell % within live cells. F CTLA4 expression (MSI) on granzyme B⁺ perforin⁺ (Grzb⁺Perf⁺) Tconv CD4⁺ T cells and Grzb⁺Perf⁺ CD8⁺ T cells. G TIGIT expression (MSI) on TEMRA Tregs, CM Tconv CD4⁺ T cells and % TIGIT⁺CD45RO⁺ Tconv within live cells. Colored dots and lines represent each subject. Box plots show mean and upper and lower quartile ranges. Significance determined by GEE. Adjusted P values are (6W vs W0) or (12W vs W0). Gating strategy shown in Fig. S2