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Table 1 Summary statistics for droplet digital PCR (ddPCR) and Illumina MiSeq results by specimen type

From: The bacterial density of clinical rectal swabs is highly variable, correlates with sequencing contamination, and predicts patient risk of extraintestinal infection

 

Water

Isolation controls

Rectal swabs

ddPCR 16S copies/sample

 Mean

14

3.14*103

9.67*107

 Median

15

3.22*103

3.17*106

 Minimum

9

1.41*103

1.18*104

 Maximum

17

5.21*103

3.23*109

 Standard deviation

2.4

1.22*103

3.41*108

 Interquartile range

3.3

1.17*103

4.63*107

Illumina MiSeq 16S reads/sample

 Mean

7.39*105

5.37*105

7.39*105

 Median

8.45*105

5.57*105

7.45*105

 Minimum

4.42*105

2.39*105

83

 Maximum

9.29*105

7.86*105

1.46*106

 Standard deviation

2.60*105

1.73*105

2.23*105

 Interquartile range

2.44*105

2.09*105

2.95*105