Fig. 5
Acute exposure of mice to TMAO significantly alters the whole brain transcriptome. A Heatmap showing expression of the 76 genes found to be significantly (PFDR < 0.1) differentially expressed in the mouse brain after 2 h exposure to 1.8 mg/kg TMAO (n = 3 per group). Data were scaled by row. B Over-representation analysis (Enrichr) showing KEGG pathways associated with the 76 genes. C Comparative analysis of significantly differentially expressed genes identified groupings associated with distinct biological functions. D Among the 197 BBB-specific genes identified in the data set, only App and Cpe were significantly (PFDR < 0.1) differentially expressed in the mouse brain after 2 h exposure to TMAO. Data are shown as mean ± s.d, n = 3 per group. Individual data points are not shown due to the negligible values of the s.d