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Fig. 3 | Microbiome

Fig. 3

From: Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)

Fig. 3

Panel a shows the relative abundances of eukaryotic groups in eight oyster samples revealed using deep-sequencing of 18S amplicons of rRNA genes. The methods are based on using non-metazoan primers (NM), blocking primers (BP), and CCSAS that combines 18S “universal” primers and cleavage using CRISPR-Cas9. Panel b shows the comparison of the number of 18S rRNA gene OTUs summed for the eight oyster samples for each microeukaryotic group. Panel c shows the Principal Coordinate Analyses (PCoA) of the community structure of the microeukaryotic 18S rRNA gene sequences in oyster spat. The PCoA ordinates the weighted Unifrac matrix that are based on the presence/absence and the relative abundance of microeukaryotic OTUs. Symbol colors represent the oyster samples, and the shapes indicate different methods (CCSAS, NM, BP)

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