Fig. 2From: A fiber-deprived diet causes cognitive impairment and hippocampal microglia-mediated synaptic loss through the gut microbiota and metabolitesDietary fiber deficiency for 15 weeks altered synaptic proteome in the hippocampus. A–E The quantitative analysis of the global proteome of the hippocampus (n = 3). A Volcano plot of differentially expressed proteins. B Analysis of GO enrichment of biological process for differentially expressed proteins. C Analysis of KEGG pathway of the differentially expressed synaptic proteins. D The heatmap of the differentially expressed synaptic proteins. E PPI network analysis of distinctly dysregulated proteins based on STRING database and Cytoscape 3.6.0. Black lines denoted the interaction between two proteins. The red node signified up-regulation, blue node signified downregulation. F–H The verification of the quantitative analysis of the global proteome of the hippocampus for downregulated proteins (CaMKIId, GAP43, and SV2C) by western blot (n = 5–6). Values are mean ± SEM. *p < 0.05 fiber deficiency (FD) group vs. control (Con) groupBack to article page