Fig. 4
From: Intestinal microbiota shapes gut physiology and regulates enteric neurons and glia
Depletion of gut microbiota stimulates neuronal Sox2 expression in the colonic myenteric plexus. a Representative immunofluorescent images of ganglia in the colonic submucosal plexus: HuC/D+ (green) and Sox2+ (magenta) cells. Scale bar: 30 μm. b Number of HuC/D+ neurons (left panel), Sox2+ cells (middle panel), and double-labelled HuC/D+/Sox2+ neurons (right panel) in the submucosal plexus of the proximal colon. c Representative immunofluorescent images of ganglia in the colonic myenteric plexus: HuC/D+ (green) and Sox2+ (magenta) cells. Arrows represent double-labelled HuC/D+/Sox2+ neurons. Scale bar: 30 μm. d Number of HuC/D+ neurons (left panel), Sox2+ cells (middle panel), and double-labelled HuC/D+/Sox2+ neurons (right panel) in the myenteric plexus of the proximal colon. Data are expressed as mean ± SEM. n = 5. *p < 0.05, **p < 0.01, ***p < 0.001; Student’s t test