Fig. 3

16S rRNA sequencing results (A–B). Distribution of A reads and B richness (alpha diversity) of 16S rRNA measured across the 13 SAF locations grouped by PMA treatment. Microbial composition of live vs dead cell communities as determined using PMA treatment (C–E). Microbial community beta diversity driven strongly by PMA treatment for C unweighted UniFrac and D weighted UniFrac (PMA- “red” and PMA+ treated “blue” samples). E Heatmap representation of the features (180 out of 1250 total genera) associated with PMA treatment as calculated using the differential abundance measure in Calour with controls labeled for reference. Comparison of microbial community composition between PMA live vs. dead treatment (F–G). Robust Aitchison PCA (RPCA) compare between PMA treated (blue) and untreated (red) SAF rooms (circles) and controls (ex) (F). Log-ratio of lowest common ancestor aggregated Pseudomonadales (order) and Bacilli (class) compared by PMA treatment between rooms (light blue) and controls (dark blue) (G). P values obtained from pairwise t tests with Bonferroni multiple comparisons correction