Fig. 5
From: Polyamine metabolism links gut microbiota and testicular dysfunction
HSP70 regulated by spermine protected against testicular toxicity induced by TP in mice and TM4 cells. a mRNA levels of HSP70s after treatment with spermine or eflornithine, a polyamine pathway inhibitor. b The function of HSP70 was indicated by cell viability, MDA, and LDH levels after treated with a HSP70 inhibitor VER155008. The concentration of spermine was 6.25 μM. c Spermine increased protein levels of HSP70s and eflornithine decreased protein levels of HSP70s. d VER155008 reduced the protective effect of spermine in mice. EF: eflornithine. The concentration of TP used in this experiment was 80 nM in TM4 cell. *P < 0.05, **P < 0.01, and ***P < 0.001 (n = 6)