Fig. 4

Unrooted phylogenetic trees of terminase large subunit (TerL) protein sequences from RefSeq prokaryotic viral genomes and soil vOTUs in the PIGEON database. Trees are color-coded by sequence source (RefSeq or soil category within PIGEON). Trees were constructed using IQ-tree and the LG+I+G4+F model of sequence evolution, using ultrafast bootstrapping and an SH-aLRT test. Bootstrap values are not displayed but can be found for each of the branches in Supplemental File 1. A Phylogenetic tree of TerL protein sequences from RefSeq prokaryotic viral genomes (n = 1613) and PIGEON soil vOTUs (n = 1011). Outer ring color represents viral family of RefSeq genomes. Phylogenetic dispersion was estimated by using Fritz & Purvis D (D). D = − 0.25 when comparing TerL sequences from RefSeq viral genomes and TerL sequences from soil vOTUs, with D < 0 indicating phylogenetic clustering. B Phylogenetic tree of TerL protein sequences from PIGEON soil vOTUs. D = 0.58 for other soil (n = 634) compared with peat, including SPRUCE (n = 377), and D = 0.41 when comparing SPRUCE (n = 51) to all other soil sequences (n = 960)