Active virus-host interactions at sub-freezing temperatures in Arctic peat soil

Microbiome

Table 1 Linkages between ¹⁸O-labeled and unlabeled viruses and bacteria in SIP incubations

Group	Description	No. of vOTU-MAG matches	Unique vOTU-MAG matches	vOTUs	MAGs
1	Active vOTU-active MAG	164	107	74	37
2	Active vOTU-unlabeled MAG	2	2	2	2
3	Unlabeled vOTU-active MAG	226	77	44	11
4	Unlabeled vOTU-unlabeled MAG	422	132	58	30
	Total	814	318	178	80

Viral operational taxonomic units (vOTUs) were linked to metagenome assembled genomes (MAGs) via nucleotide similarity and CRISPR spacers. Active vOTUs and MAGs were defined based on assimilation of ¹⁸O enriched water (heavy water) into DNA. In total, there were 814 linkages from active vOTUs to active MAGs (group 1), active vOTUs to unlabeled MAGs (group 2), unlabeled vOTUs to active MAGs (group 3), and unlabeled vOTUs to unlabeled MAGs (group 4)

ISSN: 2049-2618