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Fig. 1 | Microbiome

Fig. 1

From: Recovery of strain-resolved genomes from human microbiome through an integration framework of single-cell genomics and metagenomics

Fig. 1

Overview of the single-cell genomics and metagenomics integration framework (SMAGLinker). Single-cell sequencing reads (SRs) and metagenomic sequencing reads (MRs) are obtained from the same microbial community. (1) De novo assembly of each SR to a single-cell amplified genome (SAG). (2) SAGs of the same strain are identified into the group and co-assembled into a composite SAG (CoSAG). (3) De novo assembly of MRs into metagenome-assembled contigs (MAs). (4) MAs are classified to single-cell genome-guided bin (sgBin) by mapping MA on non-redundant SAG (nrSAG). (5) Paired nrSAGs and sgBins are merged to single-cell genome-guided MAG (sgMAG) or metagenome-guided SAG (mgSAG). (6) Unbinned contigs in MAs are extracted, and subsequently (7), re-binned and refined using conventional metagenome binning and refinement tools. (8) Four types of draft genomes (SAG, sgMAG, mgSAG, and MAG) are finally acquired

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Microbiome

ISSN: 2049-2618

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