Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption

Table 1 Composition of the experimental diets^a

Diet (g/kg)	Hca	HcaGOS	HcaInu	Lca	LcaGOS	LcaInu
Acid casein	200	200	200	200	200	200
Corn starch	241	232	239	245	237	243
Dextrose	241	232	239	245	237	243
Palm oil	160	160	160	160	160	160
Corn oil	40	40	40	40	40	40
Cellulose	60	20	20	60	20	20
Inulin	0	0	44^d	0	0	44^c
GOS	0	57.14^c	0	0	57.14^c	0
Vitamin mix^b	10	10	10	10	10	10
Mineral mix^b	35	35	35	35	35	35
CaP_i (CaHPO₄)	13.56	13.56	13.56	4.08	4.08	4.08
Total	1000	1000	1000	1000	1000	1000

^aHigh CaP_i (Hca); high CaP_i, GOS (HcaGOS); high CaP_i, inulin (HcaInu); low CaP_i (Lca); low CaP_i, GOS (LcaGOS); low CaP_i, inulin (LcaInu)
^bThe composition of the vitamin and mineral mixtures is according to the recommendation of the American Institute of Nutrition 1993 [43], except that calcium was omitted. In addition, tripotassium citrate was added instead of KH₂PO₄ and choline chloride was added instead of choline tartrate
^cTo acquire 40 g GOS/kg, the amount of GOS was increased since Vivinal GOS contains approximately 30% glucose, galactose, and lactose
^dTo acquire 40 g inulin/kg, the amount of inulin was increased since Orafti GR® inulin contains approximately 10 % glucose, fructose, and sucrose

ISSN: 2049-2618