Fig. 1
Diet-group specific average composition of microbiota in adult rats after 14 days of dietary acclimatization. Stacked bar plots of relative abundance of endogenous microbiota at A phylum level, and B family level. Alpha diversity with C Shannon diversity and D Chao1 richness as diversity indices. E Beta diversity with the average Bray-Curtis distance of each sample within each dietary group. Statistical significance between groups is tested with a Kruskal-Wallis ANOVA followed post-hoc Dunn’s multiple comparison tests. Asterisks (*) indicate significance between groups with certain p values. *p < 0.05; **p< 0.01; ***p < 0.001; ****p < 0.0001. Samples derived from experiment 1 and experiment 2 (Fig. 1F) are colored orange and green, respectively. F pRDA of the microbiota with experiment as explanatory variable (corrected for diet) showing the 10 genera with the strongest contribution to the discrimination between independent experiments. G Partial principal component analysis (PCA) (corrected for experiment) displaying the top 10 most discriminating genera, with low-CaPi samples (circles), high-CaPi samples (squares), GOS supplementation (blue), inulin supplementation (red), or un-supplemented (yellow). Sample labels for experiment 1 and experiment 2 (Fig. 1F) contain an asterisk (*) or are empty, respectively