Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome

Table 1 Kinetic parameters characterizing the thermal inactivation of xylanase 1

Temperature (°C)	K_d (min⁻¹)	t_1/2 (min)	D (min)	ΔH° (kJ∙mol⁻¹)	ΔG° (kJ∙mol−¹)	ΔS° (J∙mol⁻¹∙K⁻¹)
25	0.0017	407.73	1354.46	150.52	78.64	0.24
40	0.0180	38.51	127.92	150.40	76.58	0.24
50	0.2400	2.89	9.59	150.31	72.16	0.24
60	0.92	0.75	2.50	150.23	70.75	0.24

K_d, inactivation rate constant; t_1/2, half-time (i.e., the time after which activity is reduced to one-half of the initial value); D, the time required to reduce enzymatic activity to 10% of its original value; ΔH°, activation enthalpy; ΔG°, activation free-energy barrier; ΔS°, activation entropy of thermal denaturation

ISSN: 2049-2618