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Fig. 4 | Microbiome

Fig. 4

From: Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome

Fig. 4

Purification and functional characterization of xylanase 1. A Size-exclusion chromatography of xylanase 1 on Superdex200 (10/300) column revealing the monomeric state of the enzyme. Inset: SDS-PAGE analysis of eluted xylanase 1. B The dependence of xylanase 1 activity on pH. C Melting temperatures (Tm) of xylanase 1 determined by differential scanning fluorimetry (DSF) in different buffers (Light grey: 100 mM sodium acetate buffer: pH 4.0, 5.0, 6.0; 100 mM Tricine buffer: pH 7.0 and 8.0; Dark grey - McDougall’s buffer: pH 6.0, 7.0, and 8.0). D Michaelis-Menten plot of xylanase 1-mediated cleavage of Beechwood xylan. Xylan at the concentration range from 0.9 to 30 mg/ml was incubated with 0.05 μM of xylanase 1 in McDougall’s buffer (pH 6) at 40°C (n=3 ± standard deviation of duplicate reactions)

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Microbiome

ISSN: 2049-2618

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