Fig. 8

IL-10 increases short-chain fatty acid (SCFA) transporter expression. The human colon-derived HT-29 epithelial cell line treated with IL-10 (10 or 100 ng/mL, 24 h) displayed significant increases in the SCFA transporters MCT1, ABCG2, and HCAR2 mRNA and a reduction in MCT4 mRNA (A). The increased MCT1 mRNA was matched by a subtle increase in MCT1 protein (representative blot shown), as shown by densitometry and statistical comparisons. Panel B shows increased expression of mct1 and Abcg2 mRNA in the murine rectal epithelial CMT-93 cell line treated with il-10 (10 ng/ml, 24 h). IL-10 treatment evoked increased mct1 mRNA in primary murine colonoids (C) (10 ng/ml, 24 h) (data are mean ± SEM; data from 1 to 2 experiments; *, p < 0.05 compared to control; kDa, kilodaltons)