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Fig. 7 | Microbiome

Fig. 7

From: Cooperation between host immunity and the gut bacteria is essential for helminth-evoked suppression of colitis

Fig. 7

Helminth infection and butyrate upregulates IL-10 receptor expression. qPCR reveals increased in il-10 and il-10 receptor-α and reduced interferon (ifn)-γ in mid-colon of H. diminuta and DNBS treated mice compared to control and DNBS only treated mice. Co-treatment with broad-spectrum antibiotics (ABX), vancomycin (Van), or a mixture of polymyxin B (Pmx) and neomycin (Neo) prevented the increase in il-10 or il-10 receptor-α (rα) mRNA. Exposure to butyrate (1-10 mM; 16–24 h) significantly increased the expression of il-10rα mRNA in B a murine rectal epithelial cell line and C primary murine colonoids. Panel D is a qualitative assessment of il-10rα expression on sections of mid-colon as detected by immunocytochemistry, with representative images depicted (data are mean ± SEM; *, p < 0.05 compared to control; H. dim, mice infected with 5 H. diminuta 8 days before intra-rectal di-nitrobenzene sulphonic acid (DNBS, 3 mg, 72 h); butyrate (500 micro-L of 100 mM enema was delivered 24 and 3 h before DNBS and 24 and 48 h after DNBS) (ABX, antibiotic cocktail in drinking water ad libitum, kanamycin (40 mg/L), gentamicin (3.5 mg/L), colistin (4.2 mg/L), metronidazole (21.5 mg/L); ip. Van. at 200 μL of 0.5 mg/mL; Pmx/Neo, 1 g/L and 500 mg/L in drinking water (see Fig. 1A and suppl. Fig. 3A,D for treatment protocols)

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