Fig. 2

A Overall alpha diversity indices of ASVs and B net relatedness index (NRI) and nearest-taxon index (NTI). Statistically different treatments (pair-wise ANOVA) are connected by bracket and the level of significance is shown with *(p < 0.05), **(p < 0.01), or ***(p < 0.001). Colors represent treatments and shapes the incubation time (square—day 0, plus—day 3, cross—day 7, circle—day 14, and triangle—day 28). C Principal coordinate analysis (PCoA) using Bray-Curtis, unweighted Unifrac, and weighted Unifrac distance matrices. Ellipses represent 95% confidence interval of the standard error of the ordination points of a given grouping. Results from PERMANOVA test for each distance matrix are shown underneath each plot. Colors in B represent sampling time (red—in situ seawater at time of collection, olive green—day 0, green—day 3, blue—day 7, pink—day 14, and brown—day 28). FSC is the in-situ baseline microbial community, WAF—seawater and oil only, BEWAF—seawater, crude oil and biosurfactant, CEWAF—seawater, crude oil and synthetic dispersant, SW—seawater only, SWBS—seawater and biosurfactant, and SWD—seawater and synthetic dispersant