Fig. 5
From: Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri
L. reuteri provides protection against DSS-induced colitis, disruption of Peyer’s patches and intestinal dysbiosis. a Macroscopic analysis of the PPs surface area (mean area of PPs per mouse, mm2, n = 5 mice per group) in WT mice (control), WT mice receiving DSS (DSS, 3% in drinking water for 7 consecutive days) as well as mice receiving L. reuteri R2LC was given daily to WT mice for 14 days starting 7 days prior to DSS-treatment (L. reuteri-DSS). Histological analysis of the PPs height (μm, serially sectioned with all slides per PP analyzed, n = 5 mice per group, scale bars equal 200 μm). b Flow cytometry of live CD3-CD19+B220+ B cells, small-pre-GC-like and large-GC-like B cells in PPs (n = 6–17 mice per group). c Body weight change and disease activity index of DSS-treated mice and mice co-treated with L. reuteri (n = 9 mice per group) or FTY720 (n = 6 mice per group). d Pearson correlation of PPs B cell number and body weight loss (%) (n = 24 mice). e Bar graph depicts bacterial community composition of individual mouse, n = 4 mice per group. f–h Relative abundance of Erysipelotrichaceae, S24-7 and Lactobacillaceae in the ileal microbiota (n = 4–9 mice per group). Data are presented as median values. Contrast analysis demonstrated that bacterial taxa changed significantly with DSS-treatment (diseased P < 0.05) and was preserved by L. reuteri-treatment (protected P < 0.05). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using ANOVA with Tukey’s post hoc test