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Fig. 2  | Microbiome

Fig. 2 

From: Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

Fig. 2 

L. reuteri increases the population of Peyer’s patches B cells and enhances their effector functions. a Flow cytometry of small-pre-GC-like and large-GC-like B cell subsets from control mice or mice treated with 108 cfu L. reuteri (R2LC, 4659 or 6475) perorally for 7 consecutive days (n = 5–12 mice per group). b Numbers of S1PR1+ small pre-GC-like B cells (CD3-CD19+B220+) and their S1PR1 expression (MFI, n = 5–12 mice per group). c Illustration of enzymes regulating sphingosine-1-phosphate (S1P) homeostasis (top). q-RT-PCR analysis of gene expression (fold change, n = 6 mice per group) of the S1P pathway (bottom). d Experimental design evaluating the effect of the sphingosine-1-phosphate (S1P) receptor modulator FTY720 (top), and flow cytometry of B cell subsets (lower panels, n = 10–11 mice per group). e Principal component analysis of the microarray data, where each dot represents one sample (n = 4 samples per group). f Enrichment of gene ontology categories (BP) for genes upregulated by L. reuteri in small B cells. g Flow cytometry of DEFa3+ B cells (CD11C-CD19+B220+) in PPs and DEFa3 expression (MFI, n = 5–6 mice per group). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 using two-tailed Student’s t test

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