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Fig. 1 | Microbiome

Fig. 1

From: Commensal microbe-derived acetate suppresses NAFLD/NASH development via hepatic FFAR2 signalling in mice

Fig. 1

Prebiotic inulin supplementation prevents NAFLD/NASH development. Mice were fed a low-fat/fructose/cholesterol diet (LFC), a high-fat/fructose/cholesterol diet (HFC) or a 10% (w/w) inulin-supplemented HFC diet (HFC + IN) for 20 weeks. a Body mass of C57BL/6 mice (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7). Data are mean ± SEM. b Liver to body mass ratio (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7). c Epididymal fat to body mass ratio (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7). d Plasma cholesterol. (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7) e Plasma triglyceride (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7). f Plasma ALT (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7). g Representative plots and quantification of the flow cytometric analysis of liver mononuclear cells from LFD, HFC and HFC + IN-fed mice. CD11b+ and F4/80+ cells (LFC, n = 8; HFC, n = 7; HFC + IN, n = 7) and CD44+CD8+CD62L T cells (LFC, n = 3; HFC, n = 5; HFC + IN, n = 5) are shown. i Representative Masson’s trichrome staining of livers from LFC, HFC and HFC + IN mice. Scale bar: 50 μm; black arrows show fibrosis, black arrowheads show hepatocyte ballooning. h, j, k Quantitative PCR analysis of fibrosis-related genes in mRNA isolated from LFC, HFC and HFC + IN mouse livers(LFC, n = 8; HFC, n = 7; HFC + IN, n = 7). Each point in bf and hm represents an individual mouse (thick bars, means; error bars, SEM). The data represent at least two independent experiments with similar results. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance (ANOVA) followed by post hoc Tukey’s test)

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