Fig. 6

In-depth analysis of the temporal expression of differentially expressed carbohydrate-active enzyme (CAZymes, also known as glycosyl hydrolases (GH)) expressed genes by prokaryotes attached to fresh perennial ryegrass incubated within the rumen that differed significantly in their expression profile over rumen incubation time (line plots) and their respective taxonomic origins (bar chart below the corresponding line plot). Incubation time is indicated on the axis of the plots, i.e. T1 indicates an incubation time of 1 h. Brown bars: family Eubacteriaceae (genus Eubacterium); Pink bars: family Fibrobacteriaceae (genus Fibrobacter); Red bars: family Lachnospiraceae (genera Butyrivibrio and Pseudobutyrivibro); Blue bars: family Prevotellaceae (genus Prevotella); orange bars: Ruminococcaceae (genus Ruminococcus); Purple bars: Spirochaetaceae (genus Treponema). The significance of rumen incubation time on gene expression is indicated on each plot, with timepoint that significantly differ denoted by a different letter in the line plot