Fig. 1

Local metapangenome of SAR324 at Station ALOHA. A Pangenome. Alignment of protein orthogroups among population genomes constituting the pangenome. Population genomes without a significant amount of metagenomic reads mapped (E) have been removed to keep only locally relevant genomes. Colored bars indicate the presence of a protein into the population genome. Population genomes have been colored according to subclade/ecotype definition as suggested by ANI classification (D) and depth distribution (E). B Ordering of protein orthogroups by Ward classification of Euclidean distances between frequencies of orthogroups in SAR324 pangenome. Branches are colored according to frequencies: orthogroups from core genome (genes present in all population genomes) in red; orthogroups common to subclade A in yellow, subclade B in green, and subclades C, D, E in blue; and singletons (protein unique to a population genome) are in gray. C Square heatmap of average nucleotide identity between population genomes ordered according to ANI classification of population genomes (D). Scaled from 70 to 100% of identity. D Hierarchical ascendant classification based on the average link of ANI distance between populations genomes. E Average coverage of population genomes by metagenomic reads from depth of SAGs sampling across the water column at Station ALOHA. F Average coverage of protein orthogroups by metagenomic reads from depth of SAGs sampling across the water column at Station ALOHA