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Table 3 The approximate balance of carbon for the selected data from experiments LA and SLA based on the concentration of the media components and fermentation products (mean from three replicates shown)

From: Dynamics of dark fermentation microbial communities in the light of lactate and butyrate production

 

Experiment

Medium

 

Fermentation products

Balance 1

Experiment LA after the first passage (3 days)

200 lactate + 85 acetate

→

112 lactate + 49 butyrate + 69 acetate + 3 propionate + 4 ethanol + X (27 millimoles of carbon)**

Balance 2

Experiment LA after the second passage (6 days)

200 lactate + 85 acetate

→

8 lactate + 140 butyrate + 56 acetate + 4 propionate + 4 ethanol + X (73 millimoles of carbon)**

Balance 3

Experiment SLA after the first passage (3 days)

200 lactate + 85 acetate + 347 sucrose

→

345 lactate + 22 butyrate + 113 acetate + 250 ethanol*

Balance 4

Experiment SLA after the second passage (6 days)

200 lactate + 85 acetate + 343 sucrose

→

22 lactate + 318 butyrate + 8 acetate + 14 propionate + 46 ethanol + X (220 millimoles of carbon)**

  1. * The excess of millimoles of carbon in the fermentation products likely comes from the inoculum
  2. ** X are estimated bacterial biomass and other products including carbon dioxide