Table 2 Characteristics of the digestive liquids. The data show a mean from three replicates with ± SD, for each the analyses were performed in duplicate. Tukey’s HSD test was applied after ANOVA variance analysis to compare statistical significance; for detailed comparisons see Additional file 4

3

4.0 ± 0.0

0.8 ± 0,2

2.6 ± 0.4 ^a

0.4 ± 0.1

0.3 ± 0.5

5.5 ± 1.9

6

3.9 ± 0.1 ^#

0.8 ± 0,1

3.9 ± 0.4 ^b

0.5 ± 0.1

1.0 ± 0.1 ^$

5.6 ± 1.5 ^@

9

3.7 ± 0.1 ^##

1.0 ± 0.2

5.2 ± 0.3 ^a

0.4 ± 0.1

0.9 ± 0.6 ^$$

3.8 ± 2.1 ^@@

12

3.6 ± 0,03

1.2 ± 0.3

6.3 ± 0.7 ^{a, b}

0.5 ± 0.1

0.5 ± 0.3

3.1 ± 0.6

15

3.7 ± 0.03

1.0 ± 0.2

5.2 ± 0.7 ^a

0.3 ± 0.03

0.4 ± 0.2

4.1 ± 0.4

18

3.6 ± 0.03

1.0 ± 0.1

5.6 ± 0.2 ^{a, b}

0.5 ± 0.1

0.4 ± 0.2

4.9 ± 0.5

3

4.4 ± 0.01

1.0 ± 0.2

10.4 ± 1.5

0.5 ± 0.1

0.03 ± 0.01 ^d

6.7 ± 1.1 ^e

6

4.4 ± 0.2 ^#

0.7 ± 0.4

12.0 ± 2.1 ^{c, &}

0.6 ± 0.1

1.1 ± 1.7 ^$

5.2 ± 0.7 ^@

9

4.5 ± 0.1 ^##

0.8 ± 0.1

10.0 ± 0.5 ^&&

0.5 ± 0.1

1.1 ± 1.0 ^$$

3.4 ± 1.1 ^{e, @@}

12

4.5 ± 0.03

0.7 ± 0.2

8.3 ± 2.5

0.7 ± 0.1

1.9 ± 0.6

4.2 ± 1.5

15

4.6 ± 0.04

0.7 ± 0.2

6.3 ± 0.6 ^c

0.6 ± 0.1

3.2 ± 0.8 ^d

2.7 ± 0.6 ^e

18

4.6 ± 0.04

1.1 ± 0.3

6.8 ± 1.2 ^c

0.7 ± 0.1

3.2 ± 0.7 ^d

2.8 ± 1.1 ^e

3

4.6 ± 0.01 ^f

3.8 ± 0.4

11.2 ± 0.9

0.5 ± 0.1

0.2 ± 0.03 ^g

5.9 ± 0.6 ^h

6

5.4 ± 0.1 ^{f , #}

1.4 ± 0.1

2.5 ± 0.8 ^&

0.6 ± 0.01

7.1 ± 0.5 ^{g, $}

0.6 ± 0.04 ^{h, @}

9

5.5 ± 0.01 ^{f, ##}

2.7 ± 1.4

4.4 ± 6.5 ^&&

0.6 ± 0.02

6.5 ± 3.5 ^{g, $$}

0.9 ± 0.7 ^{h, @@}

12

4.7 ± 0.2

3.1 ± 0.9

5.2 ± 1.8

0.6 ± 0.1

4.5 ± 0.9

1.8 ± 0.8 ^h

15

5.1 ± 0.1

1.4 ± 0.6

2.9 ± 2.3

0.4 ± 0.1

5.2 ± 1.6

0.6 ± 0.1 ^h

18

4.6 ± 0.03

6.3 ± 1.8

8.8 ± 2.2

0.5 ± 0.1

2.2 ± 0.4

1.0 ± 0.1 ^h

3

6.6 ± 0.1

2.1 ± 0.5

3.4 ± 0.2 ⁱ

0.1 ± 0.04

1.5 ± 0.7 ^j

0.1 ± 0.03

6

7.0 ± 0.1 ^#

1.7 ± 0.5

0.2 ± 0.2 ^{I, &}

0.2 ± 0.1

3.5 ± 0.8 ^{j, $}

0.1 ± 0.1 ^@

9

7.2 ± 0.1 ^##

1.7 ± 0.1

0.02 ± 0.01 ^{I, &&}

0.2 ± 0.1

4.2 ± 0.2 ^{j, $$}

0.04 ± 0.04 ^@@

12

7.2 ± 0.05

1.1 ± 0.4

0.5 ± 0.3 ⁱ

0.3 ± 0.1

3.8 ± 0.7 ^j

0.2 ± 0.03

15

7.3 ± 0.03

2.1 ± 0.2

0.2 ± 0.2 ⁱ

0.2 ± 0.0

4.0 ± 0.1 ^j

0.1 ± 0.04

18

7.3 ± 0.1

1.7 ± 1.0

0.2 ± 0.15 ⁱ

0.4 ± 0.04

3.8 ± 0.5 ^j

0.1 ± 0.03

3

4.6 ± 0.03 ^k

3.4 ± 0.1

10.4 ± 0.7

0.02 ±0.01

0.9 ± 0.2 ^l

5.8 ± 1.0 ^m

6

6.2 ± 0.04 ^{k, #}

0.3 ± 0.1

0. 7 ± 1.1 ^&

0.4 ± 0.2

7.0 ± 1.3 ^{l, $}

1.1 ± 0.2 ^{m, @}

9

5.8 ±0.03 ^{k, ##}

2.2 ± 0.7

0.02 ± 0.01 ^&&

0.5 ± 0.2

8.0 ± 0.6 ^{l, $$}

0.4 ± 0.1 ^{m, @@}

12

4.7 ± 0.5

3.7 ± 0.7

4.4 ± 3.9

0.2 ± 0.1

4.4 ± 3.6

0.7 ± 0.1 ^m

15

4.8 ± 0.5

3.1 ± 2.1

6.7 ± 6.9

0.3 ± 0.2

4.7 ± 3.2

0.8 ± 0.8 ^m

18

5.1 ± 0.6

4.7 ± 2.5

5.9 ± 5.1

0.3 ± 0.1

5.4 ± 4.1

0.3 ± 0.1 ^m