The microbial metabolite p-Cresol induces autistic-like behaviors in mice by remodeling the gut microbiota

Microbiome

Table 1 Predicted proteins homologous to enzymes involved in metabolic pathways from tyrosine to p-Cresol in species related to bacterial taxa identified as upregulated both upon p-Cresol treatment and upon FMT^p-Cresol

Species	Direct pathway			Indirect pathway
	ThiH			HpdA
	Accession	% Identity	E-score	Accession	% Identity	E-score
Anaerobium acetethylicum	WP_091230597.1	38.90	3.00E− 72	WP_091236185.1	37.62	4E− 66
Duncaniella dubosii	WP_123615573.1	46.85	3.00E− 121	WP_123485485.1	32.59	5E− 13
Barnesiella sp.	MBD5257534.1	48.49	1.00E− 129	-	-	-
Barnesiella sp. An55	WP_087424377.1	49.04	3.00E− 125	-	-	-
Barnesiella intestinihominis	WP_195342987.1	48.65	2.00E− 124	-	-	-
Barnesiella viscericola	WP_025277286.1	47.95	6.00E− 123	-	-	-

Reference enzyme protein sequences from ThiH (NP_418417.1, 2-iminoacetate synthase from Escherichia coli K12, metabolizing tyrosine to p-Cresol) and HpdA (sp|Q84F14|HPDA_CLODI, 4-hydroxyphenylacetate decarboxylase activating enzyme from Clostridioides difficile, metabolizing 4-hydroxyphenylacteate to p-Cresol) were used to conduct a Blast search against sequences from the Anaerobium genus and from the Muribaculaceae family. Only sequences covering more than 50% of the query sequence and presenting amino acid identity above 30% were considered potential homologous enzymes. See also Tab. S3 (Additional file 4) for the full blast analysis

ISSN: 2049-2618