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Table 2 The comparison of fermentation incubation indexes. Significant differences between the means were determined by Tukey’s test. Differences were considered significant at P < 0.05

From: Breed differences in the expression levels of gga-miR-222a in laying hens influenced H2S production by regulating methionine synthase genes in gut bacteria

Items LB LC LT HB HC HT
pH 7.57 ± 0.08 7.49 ± 0.03 7.61 ± 0.10 7.59 ± 0.02 7.55 ± 0.05 7.48 ± 0.05
S2−, μg/g 15.06 ± 0.94a 14.83 ± 1.12a 12.50 ± 0.50b 12.32 ± 0.98b 12.99 ± 1.59ab 9.84 ± 1.44c
SO42−, mg/g 256.94 ± 29.93 268.52 ± 61.26 221.30 ± 79.04 257.87 ± 85.95 287.96 ± 46.39 243.98 ± 70.84
Acetate, mmol/L 31.53 ± 4.01 30.19 ± 0.96 32.81 ± 3.25 32.45 ± 1.85 34.63 ± 2.23 34.15 ± 2.70
Propionate, mmol/L 15.51 ± 1.54 15.34 ± 3.40 15.21 ± 1.35 16.00 ± 1.00 16.58 ± 0.91 15.59 ± 0.74
Butyrate, mmol/L 8.33 ± 0.54c 8.16 ± 0.56c 9.10 ± 0.73bc 10.81 ± 1.44a 10.67 ± 1.22a 10.17 ± 0.60ab
Total VFAs, mmol/L 55.37 ± 6.00 53.69 ± 3.18 57.11 ± 5.29 59.26 ± 2.94 61.88 ± 1.98 59.91 ± 3.70
Methionine, μg/mL 246.21 ± 18.83c 245.88 ± 15.01c 280.39 ± 5.78ab 257.70 ± 19.30 258.92 ± 12.08 299.12 ± 3.68a
  1. Data are presented as means with their standard errors
  2. Means within a row with different superscript letters differ (P < 0.05)
  3. LB Lohmann intestinal content broth added nothing, LCs Lohmann intestinal content broth added miRNA control, LT Lohmann intestinal content broth added gga-miR-222a, HB Hy-line intestinal content broth added nothing, HC Hy-line intestinal content broth added miRNA control, HT Hy-line intestinal content broth added gga-miR-222a