Fig. 6

Similar fecal microbial load but different quantitative gut microbiota profiles among the four genotype groups. (a) Microbial load (cells/g of feces) at day 0, day 21, and day 42 measured by flow cytometry (n = 8–10). (b) Genus-level fecal microbiome community variation, represented by principal coordinates analysis (Bray-Curtis dissimilarity PCoA) (n = 112). Arrows correspond to a post hoc fit of the mouse groups on the PCoA. (c) Genera displaying significant quantitative abundance differences between mouse genotypes at day 42 (n = 7–10). Genera with a prevalence across samples lower than 15% were excluded. Data are presented as the mean ± s.e.m, ^#, *P< 0.05, ^##P < 0.01, ^###, ***P < 0.001, ^####, ****P <0.0001. Green: CT ob lean mice, red: ob/ob mice, blue CT db lean mice, and violet: db/db mice. Data were analyzed by the Kruskal-Wallis test with Dunn’s multiple comparison test for (a) and (c)