Fig. 3

Different serum LPS concentration, hepatic bile acid content, and bile acid metabolism between ob/ob and db/db mice. (a) Serum LPS concentration (ng/mL) measured by competitive inhibition enzyme immunoassay. (b) Liver bile acid content (pmol/5mg tissue) quantified by HPLC-MS. (c) mRNA expression of liver bile acid synthesis and conjugation markers measured by RT-qPCR. (d) mRNA expression of liver bile acid export markers measured by RT-qPCR. (e) mRNA expression of liver bile acid reabsorption markers measured by RT-qPCR. (f) mRNA expression of ileal bile acid reabsorption markers measured by RT-qPCR. Dashed black line: CT lean mice, green: CT ob lean mice, red: ob/ob mice, blue: CT db lean mice, and violet: db/db mice. Data are presented as the mean ± s.e.m, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001(n = 8–10). For the mRNA expression, relative units were calculated versus the mean of the CT ob mice values set at 1. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. CA, cholic acid; CDCA, chenodeoxycholic acid; DCA, deoxycholic acid; MCA, muricholic acid; T, taurine; UDCA, ursodeoxycholic Acid. a, alpha; b, beta; o, omega conjugated species