Fig. 2

Different hepatic features between ob/ob and db/db mice. (a) Liver weight at necropsy (g); Total lipid content (mg lipids/mg tissue); Liver triglycerides (nmol/mg tissue); Liver cholesterol (nmol/mg tissue) measured using a spectrophotometer. (b) mRNA expression of liver lipid metabolism markers measured by RT-qPCR. (c) mRNA expression of liver immune cells markers measured by RT-qPCR. (d) Representative pictures of staining for F4/80 in the liver. Scale bar, 100 μm; magnification, × 20. Arrowheads point to crown-like structures. (e) mRNA expression of liver receptors and inflammatory cytokines markers measured by RT-qPCR. (f) mRNA expression of liver fibrosis markers measured by RT-qPCR. Green: CT ob lean mice, red: ob/ob mice, blue CT db lean mice, and violet: db/db mice. Data are presented as the mean ± s.e.m, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (n = 7–10). For the mRNA expression, relative units were calculated versus the mean of the CT ob mice values set at 1. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test