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Fig. 9 | Microbiome

Fig. 9

From: Vibrio vulnificus induces the death of a major bacterial species in the mouse gut via cyclo-Phe-Pro

Fig. 9

In vivo effects of cFP in mice. A Effect of cFP on the abundance of B. vulgatus in fecal samples. The fecal samples were collected from four-week-old female mice orally injected with cFP or PBS (n = 20 mice per each set). The total DNA extracted from each fecal sample was subjected to q-PCR using a primer set specific to B. vulgatus 16S rDNA and the universal primer set for eubacterial 16S rDNA. The relative abundance of B. vulgatus 16S rDNA was normalized with the abundance of the total bacterial 16S rDNAs, and presented by the values of 2−[CT(B.vulgatus) − CT(Total Bacteria)] with their median numbers. Statistical analysis was performed using the Student’s t-test and the resulting P-value was provided. B Effect of cFP on mouse lethality caused by V. vulnificus. Four-week-old female mice were orally injected with cFP as described above, then infected with various doses of V. vulnificus, ranging from 104 to 108 cells (n = 7 mice per each treatment) (open symbol). The same experiments were performed using the control mice, which had not been injected with cFP (closed symbol). The surviving mice were enumerated for 24 h, and their numbers were plotted against incubation time. Statistical analyses were performed using the log-rank test and the resulting P-values were provided

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