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Fig. 5 | Microbiome

Fig. 5

From: Vibrio vulnificus induces the death of a major bacterial species in the mouse gut via cyclo-Phe-Pro

Fig. 5

Cell death of B. vulgatus in the presence of cFP. A and B Live/Dead staining of B. vulgatus cells. Cells of B. vulgatus MGM001 were treated for 6 h with various concentrations of cFP ranging from 0 to 4.0 mM, and then stained with the Live/Dead cell double staining kit (A). Live and dead cells were enumerated by counting the green- and red-stained cells in at least eight fields, respectively, and their averages and standard deviations were plotted against the exposed concentrations of cFP (B). C SEM of B. vulgatus cells. B. vulgatus MGM001 was treated with 4.0 mM cFP for 6 h, then processed for SEM analysis as described in “Methods.” The length of a horizontal bar represents 1 μm. D, E, and F TEM of B. vulgatus cells. Four millimolar of cFP was added to the freshly grown cultures of three B. vulgatus strains; MGM001 (B), ATCC8482 (C), and DSM28735 (D). At 6 h post-exposure to cFP, B. vulgatus cells were processed for TEM analysis as described in “Methods.” For comparison, TEM images of the control cells (0 mM cFP) were presented in the left panels. The length of a horizontal bar represents 1 μm

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