Fig. 3

Parity is associated with significant differences in the relative abundance of key taxa at the end of gestation. Fecal samples from 18 mother sows (7 of parity zero and 11 of parities 3–7) were collected at days 37 and 114 of gestation, and shotgun metagenomic sequencing was performed. Bray-Curtis beta diversity was calculated between each sample and each high parity sample at A the beginning of gestation (day 37 of gestation) and B just prior to delivery (day 114 of gestation). All microbes with average relative abundance >1% across all 36 samples (Adj. P < 0.05) that were differentially abundant between zero and high parity animals at C day 37 and D day 114 are shown. Boxplots show the median and the first and third quartiles, with whiskers that extend to outliers up to 1.5 times the interquartile range. P values (adjusted for multiple testing in C and D and R² are shown where appropriate