Fig. 3

Temporal expression of the top 95% most highly expressed genes with a significant interaction with time involved in the KEGG methane metabolism pathway within the top 95% of expressed by prokaryotes attached to fresh perennial ryegrass incubated in situ within the rumen. Each column represents a set of genes that showed the same differential expression (DE) pattern (denoted as expression pattern on the x axis). A) Summed expression of all methane metabolism genes with the same DE pattern, in brackets the number of genes with the same DE pattern. B) The proportion of taxonomic genera contributing to the expression level for each DE pattern. C) Visual representation of the DE patterns for each set of genes across the timepoints sampled; The heatmap represents the level of expression for each timepoint (low = white, high = black); The lines and dots represent the specific DE pattern shared by all genes in this set where the timepoints connected by line and dots were not significantly different from each other. D) The level of expression of the genes from each taxonomic family across each timepoint