Fig. 2

Temporal functional and taxonomic overview of the top 90% most highly expressed genes with a significant interaction with time expressed by prokaryotes attached to fresh perennial ryegrass incubated in situ within the rumen. Each column represents a set of genes that showed the same differential expression (DE) pattern (denoted as expression pattern on the x axis). A) Summed expression level of all the genes with the same DE pattern, and in brackets is the corresponding number of genes within the same DE pattern. B) Proportion of each major functional category (FC) represented in the set of genes with the same DE pattern. C) Visual representation of the DE patterns for each set of genes across the timepoints sampled (i.e. T1 is the 1h timepoint) where: (i) the background heatmap represents the level of expression for each timepoint (low = white, high = black) and (ii) the lines and dots represent the specific DE pattern shared by all genes in this set where the timepoint dots connected by a line and do not significantly different from each other. D) The proportion of the taxonomic families contributing to the expression level for each DE pattern. E) The level of expression of the major functional categories across each timepoint