Fig. 3

Gcn5 is a repressor of autophagy. a Confocal microscopy observation on GFP-Atg8 relocalization in mycelia of the wild type, Δgcn5, and the over-expression strain OE-GCN5. Vacuoles were labeled with the CMAC dye. Bar = 10 μm. b Autophagy flux of the wild type (WT), Δgcn5, and OE-GCN5 under starvation conditions. c Transmission electron microscopy images of autophagic structures (blue arrows) in mycelia of WT, Δgcn5, and OE-GCN5 grown in CM or MM-N for 4 h. d Confocal microscopy observation on GFP-Atg8 relocalization in mycelia of OE-GCN5 or the acetyltransferase enzymatic inactive mutant OE-GCN5^E130Q. Mycelia were grown in CM or MM-N for 4 h. Bar = 10 μm. e Autophagy flux of Gcn5^WT and Gcn5^E130Q strain under starvation conditions. f Autophagy flux of the wild-type strain expressing GFP-Atg8 upon the enzymatic activity inhibitor of Gcn5, phenazine-1-carboxamide (PCN). Rapamycin was used as a positive control for autophagy induction, and the fungicide iprodione was used as a control to exclude the growth inhibition