Fig. 3

The fraction of error-free amplicon sequencing reads of different lengths using commercially available long-read sequencing technologies. Points represent observations from measurements of defined communities (the Zymo mock community) or single-strain isolates (fungal and bacterial isolates) that were reported in this manuscript for LoopSeq, and that were reported in [12] for PacBio CCS reads. The 16S rRNA and fungal 18S-ITS data are from traditional amplicon sequencing of a single genetic region (the “Methods” section). The LoopSeq genomic amplicon data is based on LoopSeq sequencing of randomly amplified regions of the genome of several bacteria (the “Methods” section). Lines represent the expected error-free fraction based on measurements of the error rates in 16S rRNA gene amplicon sequencing data, and assuming a constant per-base error rate. The Oxford Nanopore line is based on the per-base error-rate of 6% reported by the manufacturer for the R10 chemistry