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Fig. 4 | Microbiome

Fig. 4

From: Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

Fig. 4

Comparison of mortality of P. versicolora in response to lethal dsRNA upon administration by injection (injected dsRNA, I-dsRNA) or ingestion (fed dsRNA, F-dsRNA). a, d Kaplan–Meier survival curves of P. versicolora larvae (n = 30) after injection and ingestion of dsSrp54k (a) or dsActin (d). Ingestion or injection of dsGFP were performed as controls. The survival curves were analyzed by the log-rank test. b, e Expression levels of Srp54k and Actin in P. versicolora larvae fed or injected with dsSrp54k (b) or dsActin (e). c, f qRT-PCR analysis of the relative abundance of whole gut bacteria in P. versicolora larvae after injection with dsSrp54k (c) or dsActin (f) compared with dsGFP as control. P values were calculated using the independent samples t test. *** P < 0.001; NS, not significant; Different letters indicate significant difference (P < 0.05)

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