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Fig. 5 | Microbiome

Fig. 5

From: Parabacteroides produces acetate to alleviate heparanase-exacerbated acute pancreatitis through reducing neutrophil infiltration

Fig. 5

Administration of Parabacteroides alleviated acute pancreatitis in Hpa-Tg mice. a P. distasonis administration experimental design. WT and Hpa-Tg littermates were put on a course of intragastrically antibiotic cocktail administration for 3 days for gut microbiota depletion. Then mice were gavaged with P. distasonis (3 × 108 CFU/ 200 μL per mouse suspended in sterile 1490 medium) daily for 2 weeks. Equivalent sterile 1490 medium was used as vehicle control. After P. distasonis administration, 16S rRNA sequencing analysis and AP induction were performed. b Pancreas (g)/body weight (g) × 1‰. c Serum amylase. d Serum lipase. e Representative images of pancreatic H&E staining. f Histologic score. g Serum TNF-α measured by ELISA. h Serum IL-6 measured by ELISA. a–h n = 5 individuals/group. Data were expressed as mean ± SEM. Differences of data were assessed by ordinary one-way ANOVA. Exact p levels were all provided. Scale bars, 500 and 200 μm, respectively. AP, acute pancreatitis; Cn, caerulein; Hpa-Tg, heparanase-transgenic; P. distasonis, Parabacteroides distasonis; rRNA, ribosomal RNA; WT, wild type

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