Fig. 3

Transferring microbiota from Hpa-Tg mice exacerbated acute pancreatitis. a FMT experimental design. WT and Hpa-Tg littermates were put on a course of intragastrically antibiotic cocktail administration for 5 days for gut microbiota depletion prior to FMT and gavaged with the fecal contents of either WT or Hpa-Tg donor mice for 8 days. After FMT, 16S rRNA sequencing analysis and AP induction were performed. b Pancreas (g)/body weight (g) × 1‰. c Serum amylase. d Serum lipase. e Representative images of pancreatic H&E staining. f Histologic score. g Serum TNF-α measured by ELISA. h Serum IL-6 measured by ELISA. a–h n = 7 individuals/group. Data were expressed as mean ± SEM. Differences of data were assessed by ordinary one-way ANOVA or Kruskal-Wallis test depending on the sample distribution. Exact p levels were all provided. Scale bars, 500 and 200 μm, respectively. AP, acute pancreatitis; FMT, fecal microbiota transplantation; Hpa-Tg, heparanase-transgenic; rRNA, ribosomal RNA; WT, wild type