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Fig. 2 | Microbiome

Fig. 2

From: Parabacteroides produces acetate to alleviate heparanase-exacerbated acute pancreatitis through reducing neutrophil infiltration

Fig. 2

The gut microbiota differed between Hpa-Tg mice and WT mice. a–i 16S rRNA sequencing analysis in fecal bacterial DNA from healthy WT and Hpa-Tg littermates was performed. n = 15 individuals/group. a Alpha diversity (based on Chao1). b PCoA of beta diversity using Bray-Curtis metric distance. c Quantification of dissimilarity values based on (b), presented as dissimilarity values (first (box bottom), third (box top) quartiles, the median (line inside box) and 2.5 interquartile range (line ends)). d The ratio of F/B of WT and Hpa-Tg mice. e In genus level, Venn diagram of the ASVs in WT and Hpa-Tg mice. f Heatmap of selected differentially abundant features between WT and Hpa-Tg mice. g and h Cladograms generated by LEfSe indicating differences in the bacterial taxa between WT and Hpa-Tg mice. LDA score for the bacterial taxa differentially abundant between WT and Hpa-Tg mice (LDA > 3.5). Orange bars indicate taxa enrichment in WT mice, and green bars indicate taxa enrichment in Hpa-Tg mice. i The abundance of the genera Parabacteroides, Bacteroides, Prevotellaceae UCG-001, and Lachnospiraceae NK4A 136 group between WT and Hpa-Tg mice. j CRAMP and DEFB1 mRNA in intestinal tissue of healthy WT and Hpa-Tg mice. n = 6 individuals/group. Data were expressed as mean ± SEM. Differences of data between two groups were assessed by unpaired, two-tailed t test or Mann-Whitney test depending on the sample distribution. For (b and c), differences of data were assessed by ANOSIM test. Exact p levels were all provided. ANOSIM, analysis of similarities; ASVs, amplicon sequence variants; CRAMP, cathelicidin-related antimicrobial peptide; DEFB1, β-defensin 1; F/B, Firmicutes/Bacteroidetes; Hpa-Tg, heparanase-transgenic; LDA, linear discriminant analysis; LEfSe, linear discriminant analysis effect size; PCoA, principal coordinate analysis; rRNA, ribosomal RNA; WT, wild type

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