Fig. 4

The functional coverage of global human gut gene catalogs by new hGMB genomes. a The coverage of IGC [49], UHGP [8] and FUnkFams [50] by hGMB.catalog. The hGMB.catalog was constructed by extraction of 341,876 nonredundant genes from 115 newly sequenced genomes in hGMB and was BLAST analyzed against subject gene catalog IGC (pink bars), UHGP (yellow bars) and FUnkFams (green bars) with cutoff sequence identities of 40% and 60%, respectively. The y-axis names indicated the names of subject gene catalog and the sequence identities used for BLAST (in bracket). The coverage rates were listed in panel on the right side of each bar. b and c The rarefaction curves displaying the accumulatively increased coverage of the KOs (purple), GOs (blue) and unannotated genes (gray) in IGC (b) and UHGP (c) catalogs. The sampling was repeated for 50 times at each x-axis point; Light purple dot: the coverage rates of KO functions of IGC or UHGP gene catalogs when specified numbers of genomes were randomly sampled from 115 hGMB genomes; dark purple line: the mean coverage rate of KO functions; light blue dot: the coverage rates of GO functions of IGC or UHGP gene catalogs; dark blue line: the mean coverage rate of GO functions; gray dot: the coverage rates of unannotated genes of IGC or UHGP; black line: the mean coverage rate of unannotated genes of IGC or UHGP. d The rarefaction curves displaying the accumulatively increased coverage of conserved functionally unknown proteins in FUnkFams. The sampling was repeated for 50 times at each x-axis point; light green dot: the coverage rates of FUnkFams proteins when sampled randomly; dark green line: the mean value of the coverage rates