Fig. 2

Murine whole lung tissue contains increased bacterial burden relative to BAL fluid and negative controls. Whole lung tissue contains more copies of the bacterial 16S rRNA gene per mL of DNA isolated from lung or control specimens as quantified by ddPCR. Mean ± SEM and individual data points (representing the average of technical duplicates) are shown. Overall significance was determined by the Kruskal-Wallis test (p = 0.00005). Pairwise significance was determined by the pairwise Wilcoxon test and corrected for multiple comparisons using the Benjamini-Hochberg method (pairwise comparisons including whole lung or BAL fluid that are not shown were not significant). Significance key: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001