Fig. 5

Abundance of PET degradation pathway genes in PICRUSt2-assembled predicted metagenomes for all communities over time. a PET degradation pathway showing fold change for KEGG orthologs within treatments compared with the no carbon control (the fold change for the inoculum was calculated against the mean of the no carbon controls across all time points). b Taxonomic contributions to each KEGG ortholog involved in PET degradation. Taxonomic contributions shown are scaled by the relative abundance of each taxon as well as the number of gene copies possessed by that taxon. All taxa with a total contribution below 0.5% are grouped in ‘Other’. All fold changes and relative abundances shown are means of biological replicates (n=3). See Table S2 for individual ASVs, Table S13 for details of the PETases predicted and Table S14 for Nearest Sequenced Taxon Index (NSTI) values for all time points. Figure S7 shows the predicted abundance of genes for the potential alternative pathways for PET degradation highlighted by the Thioclava sp. BHET1 proteomic analysis