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Fig. 5 | Microbiome

Fig. 5

From: Microbial community characterization of shrimp survivors to AHPND challenge test treated with an effective shrimp probiotic (Vibrio diabolicus)

Fig. 5

Identification of PirVP genes in isolated colonies from ILI strain. a PCR characterization of ILI’s individual colonies (1-23) and positive control (lane 24; V. parahaemolyticus BA94C2). PCR targets the PirVP genes coded in the plasmid from BA94C2. Notice that all strains were positive for the plasmid except two (lanes 20 and 21). EPM: DL2000 DNA marker. b Shrimp cumulative mortality after being challenged with transconjugant colonies of the ILI strain and V. parahaemolyticus BA94C2 strains. Negative control was TSB 2% NaCl. Bars indicate standard deviations (n = 5 replicates, see methods for the number of shrimps per assay). c ILI’s individual colonies recovered from hepatopancreas and stomachs of surviving shrimp challenged with transconjugant colonies of the ILI strain. Lane 1: V. parahaemolyticus BA94C2 containing the PirVP genes; lanes 2–8: ILI’s individual colonies; lane 9: V. parahaemolyticus BA94C2 containing the PirVP genes; lane 10: ILI colony not containing the PirVP genes; and lane 11: PCR negative control; EPM: DL2000 DNA marker

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Microbiome

ISSN: 2049-2618

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