Fig. 3

Microbiome dynamics during larval diapause. a Bacterial titres in non-diapausing larvae, their age-matched early diapause siblings and in diapausing larvae over a 6-month timecourse. Each datapoint represents a pool of 5–12 larvae (Suppl. Table 3). Different letters indicate significant differences between experimental conditions based on Kruskal-Wallis rank sum test and Dunn’s post hoc test for multiple comparisons. b Microbiome composition per experimental condition. The most abundant bacterial genera are represented in the legend. Microbiome composition in each replicate sample is provided in Supplementary Figure S1. c Principal Coordinates Analysis based on unweighted unifrac distances showing differences in microbiome composition depending on diapause. The datapoints correspond to the same sibling pools as in (a). d Nonmetric Multidimensional Scaling based on Bray-Curtis distances between bacterial communities demonstrating the correlation between changes in microbiome composition and environmental factors, i.e., temperature and host nutrient levels. Datapoints are colour-coded by experimental condition as in (c)