Fig. 1

Experimental design. Diagram illustrating the “switch” to diapause induction in female N. vitripennis exposed to short photoperiods. This switch was leveraged to obtain final instar non-diapausing larvae and their age-matched diapause-destined siblings. The latter were then used in a diapause timecourse experiment. Coloured circles indicate which experiments were performed at each time-point. Diapause larvae in the picture of a mixed clutch are labelled with “D”