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Fig. 5 | Microbiome

Fig. 5

From: High throughput sequencing provides exact genomic locations of inducible prophages and accurate phage-to-host ratios in gut microbial strains

Fig. 5

Detection of inducible prophages in ex vivo-cultured murine faecal samples. (a) Mean OPR counts (upper panel) and ΔPtoH ratio shown as l2fc of genes in the Flavonifractor plautii YL31 genome (lower panel) from  mitomycin C-induced versus control cultures of murine, faecal pellets reveal two inducible prophages. The areas highlighted in grey indicate genomic regions of prophage locations that are shown in panels b and c. (b/c) YL31 phage 1 and YL31 phage 2 show mean OPR counts (upper panels), ΔPtoH ratio (middle panels) and in silico-predicted regions of prophages in the reference genome of F. plautii YL31 (lower panel) using Prophage Hunter, PHASTER, VIBRANT and VirSorter. Visualisations include prophage predictions of category 1 and 2 for VirSorter, and prophages predicted to be intact or active for PHASTER and Prophage Hunter and predicted prophage of VIBRANT, respectively. None of the prediction tools was able to predict unambiguously start and end coordinates of induced prophages. Statistical significance of differences between induced (n=3) and control (n=3) cultures of F. plautii YL31 (a-c) was tested as described in the legend of Fig. 3

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Microbiome

ISSN: 2049-2618

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