Table 1 Primers used for RT-qPCR of endogenous reference gene and target genes
From: Cross-kingdom inhibition of bacterial virulence and communication by probiotic yeast metabolites
Gene | Function | Forward sequences (5′–3′) | Reverse sequences (5′–3′) |
|---|---|---|---|
mdh | Regulate malate dehydrogenase | CTGGCGGCATTGGTCAAGCCC | ACCCGGTGTGACAGGCGCAA |
vpsT | Vibrio polysacchaired transcriptional regulator | CGCAGTATTCAGATGCTGGTG | GACCTCTTTCGCATCAGGACA |
ctxA | Cholerae toxin subunit A | AGCAGTCAGGTGGTCTTATGC | CCCGTCTGAGTTCCTCTTGC |
aphA | Virulence gene regulator | ACCGGGTACGATATAACCAAAGAG | GATGGCTGGCTTTCCAGAAG |
toxT | Transcription activator of virulence genes | TGACGCATACCCATCGACAG | TCACCAGCTAAAAGCCGAGC |
tcpH | Toxin-co-regulated pilus | TGTTTGGCTTACCCAGACCG | TTCTGAGAGCTAGGATCTGGC |
tcpP | Toxin-co-regulated pilus | ATTGCATATCAGTCTGGGTTTGC | TCACTTGGTGCTACATTCATGG |